25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner
Journal of neuroinflammation
Open Access Publication
Rights and Permissions
Wong, M.Y., Lewis, M., Doherty, J.J. et al. 25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner. J Neuroinflammation 17, 192 (2020). https://doi.org/10.1186/s12974-020-01869-3 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Wong, Man Ying; Lewis, Michael; Doherty, James J; Shi, Yang; Cashikar, Anil G; Amelianchik, Anna; Tymchuk, Svitlana; Sullivan, Patrick M; Qian, Mingxing; Covey, Douglas F; Petsko, Gregory A; Holtzman, David M; Paul, Steven M; and Luo, Wenjie, "25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner." Journal of neuroinflammation. 17, 1. 192 (2020).
Additional file 1: Figure S1. a). Expression of CH25H, Cyp27a1 and Cyp7b1 in different cell types in brain based on the Stanford transcriptome database generated by Barres and colleagues (http://www.brainrnaseq.org). b). GC-MS analysis of 25-HC levels in the conditioned medium (left) and total protein levels of cell lysate of primary mouse microglia from wild-type and CH25H-/- mice treated with LPS (0, 0.1, 1, 10, 100, 1000ng/ml). Figure S2a. Differential expression of CH25H or its ortholog in a comparison within one of the datasets. Fold-Changes are relative to non-transgenic, untreated, normal, adult, cortical or parenchymal microglia as appropriate, or, for the last two comparisons, relative to non-myeloid CNS cells (Friedman, et al Cell Report, 2018) http://researchpub.gene.com/BrainMyeloidLandscape. 2b. Relative CH25H gene expression in nonphagocytic (NP) and phagocytic (P) wild-type (WT) or apoe-/- (KO) microglia by RNA seq analysis data generated by Krasemann et al, (Immunity, 47:566, 2017). Figure S3. A schematic diagram for the mechanism associated with 25-HC in amplifying IL-1b production via inflammasome activation.