Error-corrected sequencing strategies enable comprehensive detection of leukemic mutations relevant for diagnosis and minimal residual disease monitoring
BMC Medical Genomics
Open Access Publication
Rights and Permissions
Crowgey, E.L., Mahajan, N., Wong, W.H. et al. Error-corrected sequencing strategies enable comprehensive detection of leukemic mutations relevant for diagnosis and minimal residual disease monitoring. BMC Med Genomics 13, 32 (2020). https://doi.org/10.1186/s12920-020-0671-8 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Crowgey, Erin L; Mahajan, Nitin; Wong, Wing Hing; Gopalakrishnapillai, Anilkumar; Barwe, Sonali P; Kolb, E Anders; and Druley, Todd E, "Error-corrected sequencing strategies enable comprehensive detection of leukemic mutations relevant for diagnosis and minimal residual disease monitoring." BMC Medical Genomics. 13, 1. 32 (2020).
Figure S1. Bioinformatics utilities for variant detection.
12920_2020_671_MOESM2_ESM.jpg (759 kB)
Figure S2. Schema of error-corrected sequencing molecules.
12920_2020_671_MOESM3_ESM.jpg (695 kB)
Figure S3. Graphical representation of a CNV loss in CBL.
12920_2020_671_MOESM4_ESM.jpg (762 kB)
Figure S4. RNA-ECS is accurate to single transcripts without normalization.
12920_2020_671_MOESM5_ESM.xlsx (9 kB)
Table S1. Summary of demographics for the pediatric leukemia samples.
12920_2020_671_MOESM6_ESM.xlsx (11 kB)
Table S2. Genes targeted on the DNA and RNA panels.
12920_2020_671_MOESM7_ESM.xlsx (16 kB)
Table S3. Structural variants identified via RNA-ECS in primary diagnostic samples.