Journal
Elife
Publication Date
1-12-2022
Volume
11
First Page
e72668
Document Type
Open Access Publication
DOI
10.7554/eLife.72668
Rights and Permissions
eLife 2022;11:e72668 DOI: 10.7554/eLife.72668. © 2022, Reichenbach et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Recommended Citation
Reichenbach, Alex; Kravitz, Alexxai V.; and et al, "Metabolic sensing in AgRP neurons integrates homeostatic state with dopamine signalling in the striatum." Elife. 11, e72668 (2022).
https://digitalcommons.wustl.edu/open_access_pubs/11329
elife-72668-fig1-data1-v3.zip (589 kB)
Figure 1—source data 1 Deletion of Crat in AgRP neurons is a model of impaired metabolic sensing.
elife-72668-fig2-data1-v3.zip (739 kB)
Figure 2—source data 1 Impaired metabolic sensing in AgRP neurons affects dopamine release in the nucleus accumbens.
elife-72668-fig3-data1-v3.zip (169 kB)
Figure 3—source data 1 Impaired metabolic sensing in AgRP neurons affects motivation for sucrose rewards during fasting.
elife-72668-fig4-data1-v3.zip (810 kB)
Figure 4—source data 1 Impaired metabolic sensing in AgRP neurons reduces dopamine release in the nucleus accumbens during a progressive ratio session.
elife-72668-fig5-data1-v3.zip (717 kB)
Figure 5—source data 1 The effect of impaired metabolic sensing in AgRP neurons on dopamine release in the dorsal striatum.
elife-72668-fig6-data1-v3.zip (55 kB)
Figure 6—source data 1 Impaired metabolic sensing in AgRP neurons does not affect dopamine release in the dorsal striatum during a progressive ratio session.
elife-72668-sa2-fig1-v3.jpg (9 kB)
Author response image 1
elife-72668-transrepform1-v3.docx (111 kB)
Transparent reporting form
elife-72668-video1.mp4 (2652 kB)
GCaMP6s AgRP neural activity time locked to behaviour at 10 x normal speed in an ad libitum-fed WT mouse previously exposed to a peanut butter pellet. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video2.mp4 (3596 kB)
GCaMP6s AgRP neural activity time locked to behaviour at 10 x normal speed in an ad libitum-fed KO mouse previously exposed to a peanut butter pellet. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video3.mp4 (8442 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in an ad libitum-fed WT mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video4.mp4 (7829 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in a fasted WT mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video5.mp4 (15089 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in an ad libitum-fed KO mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video6.mp4 (8636 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in a fasted KO mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
Figures and figure supplements.pdf (11230 kB)
Figure 1—source data 1 Deletion of Crat in AgRP neurons is a model of impaired metabolic sensing.
elife-72668-fig2-data1-v3.zip (739 kB)
Figure 2—source data 1 Impaired metabolic sensing in AgRP neurons affects dopamine release in the nucleus accumbens.
elife-72668-fig3-data1-v3.zip (169 kB)
Figure 3—source data 1 Impaired metabolic sensing in AgRP neurons affects motivation for sucrose rewards during fasting.
elife-72668-fig4-data1-v3.zip (810 kB)
Figure 4—source data 1 Impaired metabolic sensing in AgRP neurons reduces dopamine release in the nucleus accumbens during a progressive ratio session.
elife-72668-fig5-data1-v3.zip (717 kB)
Figure 5—source data 1 The effect of impaired metabolic sensing in AgRP neurons on dopamine release in the dorsal striatum.
elife-72668-fig6-data1-v3.zip (55 kB)
Figure 6—source data 1 Impaired metabolic sensing in AgRP neurons does not affect dopamine release in the dorsal striatum during a progressive ratio session.
elife-72668-sa2-fig1-v3.jpg (9 kB)
Author response image 1
elife-72668-transrepform1-v3.docx (111 kB)
Transparent reporting form
elife-72668-video1.mp4 (2652 kB)
GCaMP6s AgRP neural activity time locked to behaviour at 10 x normal speed in an ad libitum-fed WT mouse previously exposed to a peanut butter pellet. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video2.mp4 (3596 kB)
GCaMP6s AgRP neural activity time locked to behaviour at 10 x normal speed in an ad libitum-fed KO mouse previously exposed to a peanut butter pellet. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video3.mp4 (8442 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in an ad libitum-fed WT mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video4.mp4 (7829 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in a fasted WT mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video5.mp4 (15089 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in an ad libitum-fed KO mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
elife-72668-video6.mp4 (8636 kB)
GRAB-DA activity (dopamine release) time locked to behaviour at 10 x normal speed in a fasted KO mouse. The mouse is first exposed to wood dowel, followed by chow, followed by a ~ 70 mg PB chip. The video shows raw data collected in mV at the photoreceiver prior to df/f calculations.
Figures and figure supplements.pdf (11230 kB)
