Publication Date




First Page


Document Type

Open Access Publication



Figures and figure supplements.pdf (670 kB)
elife-73577-fig3-data1-v2.xlsx (12 kB)
Figure 3—source data 1 PCR validation of the candidate DNMs found by the various pipelines during the Mutationathon. TP means validated as true positive DNM and FP appeared as false positive. The genotypes of all individuals as shown by the PCR validation are presented.

elife-73577-fig3-data2-v2.pdf (2083 kB)
Figure 3—source data 2 Sanger sequencing chromatograms of the 39 DNM candidate sites that were successfully amplified for the four individuals, i.e. father (Noot), mother (M), offspring (Heineken), and second-generation offspring (Hoegaarde). For each alignment, the candidate germline mutation position is located under the black square. The last six chromatograms (surrounded by red boxes) are the candidates that were detected as false-positive candidates.

elife-73577-fig4-data1-v2.xlsx (7 kB)
Figure 4—source data 1 Number of candidate DNMs, estimated callable genome and per generation mutation rate by each researcher group.

elife-73577-fig5-data1-v2.xlsx (9 kB)
Figure 5—source data 1 Details on the number of candidate DNMs, the number of false positive calls, the size of the callable genome, the false negative rate and the final estimated mutation rate using various individual filters.

elife-73577-supp1-v2.docx (55 kB)
Supplementary file 1 Four supplementary tables with details on the methods used in the literature, Genome Analysis ToolKit (GATK) site filters, site-specific and sample-specific filters used in the literature, and the PCR experiment.

elife-73577-transrepform1-v2.docx (108 kB)
Transparent reporting form