Open Access Publication
Suk Lee, Kyung; Balci, Hamza; Jia, Haifeng; Lohman, Timothy H.; and Ha, Taekjip, ,"Direct imaging of single UvrD helicase dynamics on long single-stranded DNA." Nature Communications.4,. 1878. (2013).
Supplementary Figures S1-S8, Supplementary Methods and Supplementary References
ncomms2882-s2.avi (282 kB)
A fluorescence movie showing translocation of UvrD on a long ssDNA in real time. The ssDNA molecule is not visible but laid vertically in the center of the movie, with its 3′-end at the top and its 5′-end at the bottom. Raw data from an EMCCD camera (2 bytes per pixel) were converted to 8-bit color images for better contrast. Beware that some video renderers automatically smoothen the movie.
ncomms2882-s3.avi (683 kB)