Journal of Neuroinflammation
Open Access Publication
Chuang, Dennis Y.; Simonyi, Agnes; Kotzbauer, Paul T.; Gu, Zezong; and Sun, Grace Y., ,"Cytosolic phospholipase A 2 plays a crucial role in ROS/NO signaling during microglial activation through the lipoxygenase pathway." Journal of Neuroinflammation.12,. 199. (2015).
High concentrations of LPS and IFNγ were toxic to primary microglia at 24 h post-stimulation.
s12974-015-0419-0-s2.tif (5568 kB)
cPLA 2 protein expression level decreased significantly after siRNA knockdown.
s12974-015-0419-0-s3.tif (9860 kB)
NO/ROS production by BV-2 cells after IFNγ stimulation was mitigated by LOX-12/15 inhibition, but not by LOX-5 inhibition.