Open Access Publication
Gish, Stacey R.; Maier, Ezekiel J.; Haynes, Brian C.; Santiago-Tirado, Felipe H.; Srikanta, Deepa L.; Ma, Cynthia Z.; Li, Lucy X.; Williams, Matthew; Crouch, Erika C.; Khader, Shabaana A.; Brent, Michael R.; and Doering, Tamara L., ,"Computational analysis reveals a key regulator of Cryptococcal virulence and determinant of host response." mBio. 7,2. e00313-16. (2016).
Capsule thickness of cell populations.
mbo002162760st2.pdf (65 kB)
Gene expression in cells overexpressing USV101.
mbo002162760st3.pdf (54 kB)
Monosaccharide composition of GXM from the indicated strains in mole percent.
mbo002162760st4.pdf (76 kB)
Fold change in gene expression of the usv101Δ mutant compared to the wild type.
mbo002162760s1.pdf (156 kB)
mbo002162760sf2.pdf (1782 kB)
Phenotypes of usv101Δ cells and controls
mbo002162760sf3.pdf (248 kB)
Percentage of Usv101’s direct targets that are repressed (blue, left-hand scale) and the concentration of USV101 mRNA (red, right-hand scale) at 0, 1.5, and 24 h after shifting to capsule induction conditions.
mbo002162760sf4.pdf (393 kB)
Capsule thickness as a function of GAT201 expression (determined by RNA-seq).
mbo002162760sf5.pdf (253 kB)
Fold-increase in colony-forming units (CFU) of the indicated strains in lung homogenates 1 week after intranasal inoculation of 6-week-old female C57/Bl6 mice, assessed as in reference 8.
mbo002162760sf1.pdf (228 kB)
Relative expression of USV101 plotted against capsule thickness for cells grown under a variety of different capsule-inducing conditions (blue symbols) (7) and the line that best fits the data (red).