Open Access Publication
Chen, Christopher Phillip; Zhang, Jiehua; Zhang, Bin; Hassan, Mohamed G; Hane, Kyle; Chen, Caroline C; Navarro Palacios, Ana Alejandra; Kapila, Sunil; Jheon, Andrew H; and Goodwin, Alice F, "Unilateral Loss of Maxillary Molars in Young Mice Leads to Bilateral Condylar Adaptation and Degenerative Disease.." JBMR Plus. 6, 7. e10638 (2022).
Supplemental Fig. S1. Experimental set up for tooth extractions. Mouse was placed in a stabilizing holder and a mouth prop and cheek retractors were used to access the right maxillary molar teeth for extraction.
jbm410638-sup-0002-figures2.pdf (1488 kB)
Supplemental Fig. S2. Cranium landmarks. (A) Dorsal and (A') ventral views of the isosufaces of the cranium with landmarks utilized for the study marked by numbered green dots.
jbm410638-sup-0003-figures3.pdf (284 kB)
Supplemental Fig. S3. Unilateral molar extraction results in significant bilateral mandibular shape changes. (A) Principal component analysis (PCA) comparing both right and left mandibles shows that the control (in blue) and experimental (in red) samples separated along PC1 and PC2. (B) Wireframes showing average (in gray), PC1 Minimum (Min; in blue), and PC1 Maximum (Max; in red) of right (solid line) and left (dashed line) hemi-mandibles. (B′) Representative isosurfaces of control (PC1 Min) and experimental (PC1 Max) mandibles.
jbm410638-sup-0004-figures4.pdf (708 kB)
Supplemental Fig. S4. The alveolar height of the right mandible in the extraction mice is significantly increased compared to control. (A) Linear measurements of the alveolar height in the right extraction mandible showed significant increase in the experimental mice compared to control (*p < 0.003). (B) There was no significant difference in alveolar height in the left mandible between control and extraction mice.
jbm410638-sup-0005-figures5.pdf (735 kB)
Supplemental Fig. S5. Canonical variate analysis on mandibles and condyles in extraction mice compared to control. (A–C) Canonical variate analysis shows clear separation between both mandibles (A), the right/extraction mandible (B), the left/non-extraction mandible (C), the right/extraction condyle (D), and the left/non-extraction condyle (E) in control (blue) and experimental (red) samples.
jbm410638-sup-0006-figures6.pdf (662 kB)
Supplemental Fig. S6. No clear sex differences are observed in control or experimental samples. (A) Principal component analysis (PCA) showed the male and female samples did not cluster in the control or experimental groups, suggesting the shape differences observed due to tooth extraction did not differ significantly between males and females.
jbm410638-sup-0007-supinfo.docx (19 kB)
Supplemental Materials and Methods
jbm410638-sup-0008-tables.docx (15 kB)
Supplemental Table S1. Probability values from statistical hypothesis tests using calculated centroid size and Procrustes distance. Bold indicates p < 0.05. Supplemental Table S2. Modified Mankin scoring system utilized.