Optimized Legionella expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant

Authors

Sukhithasri Vijayrajratnam, Washington University School of Medicine in St. Louis
Jonasz B Patkowski, Imperial College London
Joshua Khorsandi, Johns Hopkins University
Wandy L Beatty, Washington University School of Medicine in St. Louis
Shanmugapriya Kannaiah, Washington University School of Medicine in St. Louis
Ahmet Hasanovic, Washington University School of Medicine in St. Louis
Tamara J O'Connor, Johns Hopkins University
Tiago R D Costa, Imperial College London
Joseph P Vogel, Washington University School of Medicine in St. Louis

Journal

Microbiology Spectrum

Publication Date

7-1-2025

Volume

13

Issue

7

First Page

e0322224

Document Type

Open Access Publication

DOI

10.1128/spectrum.03222-24

Rights and Permissions

Vijayrajratnam S, Patkowski JB, Khorsandi J, Beatty WL, Kannaiah S, Hasanovic A, O'Connor TJ, Costa TRD, Vogel JP. Optimized Legionella expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant. Microbiol Spectr. 2025 Jul;13(7):e0322224. doi: 10.1128/spectrum.03222-24. © 2025 Vijayrajratnam et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Recommended Citation

Vijayrajratnam, Sukhithasri; Patkowski, Jonasz B; Khorsandi, Joshua; Beatty, Wandy L; Kannaiah, Shanmugapriya; Hasanovic, Ahmet; O'Connor, Tamara J; Costa, Tiago R D; and Vogel, Joseph P, "Optimized Legionella expression strain for affinity purification of His-tagged membrane proteins eliminates major multimeric contaminant." Microbiology Spectrum. 13, 7. e0322224 (2025).
https://digitalcommons.wustl.edu/oa_4/5552

Additional Links

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