Journal
Molecular Biology of the Cell
Publication Date
3-1-2021
Volume
32
Issue
5
First Page
413
Last Page
421
Document Type
Open Access Publication
DOI
10.1091/mbc.E20-04-0241
Rights and Permissions
Onken MD, Blumer KJ, Cooper JA. Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium. Mol Biol Cell. 2021 Mar 1;32(5):413-421. doi: 10.1091/mbc.E20-04-0241. © 2021 Onken et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). https://www.molbiolcell.org/doi/10.1091/mbc.E20-04-0241.
Recommended Citation
Onken, Michael D.; Blumer, Kendall J.; and Cooper, John A., "Uveal melanoma cells use ameboid and mesenchymal mechanisms of cell motility crossing the endothelium." Molecular Biology of the Cell. 32, 5. 413 - 421. (2021).
https://digitalcommons.wustl.edu/open_access_pubs/10133
Representative DIC movies of UM cells added to endothelial monolayers after treatment with vehicle control (DMSO) or FR900359. 20 sec intervals between frames; 2 hrs total elapsed time.
Movie S2.mov (826 kB)
Representative DIC movies of UM cells added to endothelial monolayers after treatment with ROCK inhibitor (Y27632) or myosin inhibitor (Blebbistatin). 20 sec intervals between frames; 2 hrs total elapsed time.
Movie S3.mov (816 kB)
Representative DIC movies of UM cells added to endothelial monolayers after treatment with Rac inhibitor (NSC23766) or PAK inhibitor (IPA3). 20 sec intervals between frames; 2 hrs total elapsed time.
Movie S4.mov (804 kB)
Representative DIC movies of UM cells added to endothelial monolayers after treatment with Arp2/3 inhibitor (CK666). Left panel shows incomplete intercalation; right panel shows direct transmigration without intercalation. 20 sec intervals between frames; 2 hrs total elapsed time.
movie S5.mov (816 kB)
Representative DIC movies of BAP1+/- 92.1 UM subclones, 2D3 and 1D7, added to endothelial monolayers. 20 sec intervals between frames; 2 hrs total elapsed time.
