Journal
Elife
Publication Date
4-7-2021
Volume
10
First Page
e63288
Document Type
Open Access Publication
DOI
10.7554/eLife.63288
Rights and Permissions
eLife 2021;10:e63288 DOI: 10.7554/eLife.63288. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Recommended Citation
Chadda, Rahul; Bernhardt, Nathan; Kelley, Elizabeth G.; Teixeira, Susana C.M.; Griffith, Kacie; Gil-Ley, Alejandro; Öztürk, Tuğba N.; Hughes, Lauren E.; Forsythe, Ana; Krishnamani, Venkatramanan; Faraldo-Gómez, José D.; and Robertson, Janice L., "Membrane transporter dimerization driven by differential lipid solvation energetics of dissociated and associated states." Elife. 10, e63288 (2021).
https://digitalcommons.wustl.edu/open_access_pubs/10393
Figure 2—source data 1. CGMD simulation specifications.
elife-63288-fig2-data2-v2.zip (7441 kB)
Figure 2—source data 2 Simulation files for CLC-ec1 in 2:1 POPE:POPG.
elife-63288-fig3-data1-v2.docx (155 kB)
Figure 3—source data 1. Differential scanning calorimetry for mixed DL/PO 2:1 PE/PG membranes.
elife-63288-fig3-data2-v2.docx (156 kB)
Figure 3—source data 2. SANS bilayer thickness analysis for mixed DL/PO 2:1 PE/PG membranes.
elife-63288-fig3-data3-v2.zip (5837 kB)
Figure 3—source data 3 Simulation files for DL/PO membranes.
elife-63288-fig4-data1-v2.docx (157 kB)
Figure 4—source data 1. Cryo-EM radii of 20% DL 2:1 POPE/POPG
elife-63288-fig4-data2-v2.docx (156 kB)
Figure 4—source data 2. Chloride transport of CLC-ec1-Cy5 in DL/PO proteoliposomes.
elife-63288-fig4-data3-v2.docx (156 kB)
Figure 4—source data 3. Photobleaching data for monomeric control CLC-ec1 I201W/I422W, WW-Cy5 in 20% DL, 80% PO, 2:1 PE/PG lipids.
elife-63288-fig4-data4-v2.docx (156 kB)
Figure 4—source data 4. Photobleaching data for dimeric control CLC-ec1 R230C/L249C, RCLC-Cy5 in 20% DL, 80% PO, 2:1 PE/PG lipids.
elife-63288-fig4-data5-v2.docx (156 kB)
Figure 4—source data 5. Photobleaching data for monomeric control CLC-ec1 WT-Cy5 in 20% DL, 80% PO, 2:1 PE/PG lipids.
elife-63288-fig4-data6-v2.docx (156 kB)
Figure 4—source data 6. Shift in dimer equilibrium upon fusion with DL containing vesicles.
elife-63288-fig4-data7-v2.xlsx (10 kB)
Figure 4—source data 7. Cy5 labeling yields.
elife-63288-fig5-data1-v2.docx (158 kB)
Figure 5—source data 1. Photobleaching titration of CLC-ec1 WT-Cy5 in 2:1 PE/PG mixed DL/PO membranes.
elife-63288-fig5-data2-v2.docx (155 kB)
Figure 5—source data 2. Testing for DL contamination during dialysis.
elife-63288-fig5-data3-v2.docx (158 kB)
Figure 5—source data 3. DL and PO concentrations, mole fraction and molality in the titrated DL/PO 2:1 POPE/POPG lipid bilayers.
elife-63288-fig6-data1-v2.zip (14379 kB)
Figure 6—source data 1. Simulation files for CLC-ec1 in DL/PO membranes.
Figures and figure supplements.pdf (12465 kB)
Transparent Reporting Form.pdf (130 kB)
