Journal
Elife
Publication Date
2021
Volume
10
First Page
e65952
Document Type
Open Access Publication
DOI
10.7554/eLife.65952
Rights and Permissions
eLife 2021;10:e65952 DOI: 10.7554/eLife.65952. © 2021, Kweon et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Recommended Citation
Kweon, Hyae Yon; Jeong, Se-Jin; and et al, "Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway." Elife. 10, e65952 (2021).
https://digitalcommons.wustl.edu/open_access_pubs/10866
elife-65952-fig3-data1-v3.pdf (5157 kB)
Figure 3—source data 1 Identification of a potential Naa10 homolog.
elife-65952-fig4-data1-v3.pdf (202 kB)
Figure 4—source data 1 Characterization of a potential Naa10 homolog.
elife-65952-fig5-data1-v3.pdf (28 kB)
Figure 5—source data 1 Generation of Naa12KO mice.
elife-65952-supp1-v3.docx (76 kB)
Tables related to genotypes, phenotypes and Mendelian ratios. (a) Genotypes of offspring from Naa10+/- female mice crossed to the Naa10+/Y male mice. Expected and observed Mendelian ratio of genotypes in offspring at E10.5, E13.5, E18.5 and adults from crosses of Naa10+/- female and Naa10+/Y male mice. The percentage of adult Naa10-/Y mice significantly decreases. (b) Genotypes of offspring from Naa10+/tm1a female mice crossed to the Naa10+/Y male mice. Expected and observed Mendelian ratio of genotypes in offspring at E10.5, E13.5, E18.5 and adults from crosses of Naa10+/tm1a female and Naa10+/Y male mice. The percentage of adults Naa10tm1a/Y mice significantly decreases. (c) Cervical fusion skeletal analyses in Naa10 knockout (KO) mice. (d) Matings and litter size analyses. (e) Genotypes of offspring from Naa12+/- female mice crossed to the Naa12+/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (f) Genotypes of offspring from Naa10+/- Naa12+/+ female mice crossed to the Naa10+/y Naa12+/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (g) Genotypes of offspring from Naa10+/- Naa12+/+ female mice crossed to the Naa10+/y Naa12-/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (h) Mendelian and observed offspring distributions from Naa10(+/Y); Naa12(+/-) male and Naa10(+/-); Naa12(+/-) female breeding. (i) Mendelian and observed offspring distributions from Naa10(+/Y); Naa12(-/-) male and Naa10(+/-); Naa12(+/-) female breeding. (j) Mendelian and observed postnatal offspring distributions from Naa10(+/Y); Naa12(+/-) male and Naa10(+/-); Naa12(+/+) female breeding. (k) Mendelian and observed postnatal offspring distributions from Naa10(+/Y); Naa12(-/-) male and Naa10(+/-); Naa12(+/+) female breeding. (l) Mendelian and observed age-specific offspring distributions from four crosses. (m) Mendelian and observed cumulative offspring distributions from all four crosses. (n) Mice analyzed by weighing, according to genotype. (o) Effects of Naa10 KO on growth rate of Naa10 mice on pure genetic background. (p) Effects of Naa10 and Naa12 KOs on growth rate on mixed genetic background.
elife-65952-supp2-v3.xlsx (8412 kB)
Mass spectrometry analyses. (a) N-termini detected in mouse embryonic fibroblasts (MEFs) (Excel sheet). (b) N-termini detected in all MEF samples (Excel sheet). (c) Header Key, showing the abbreviation meanings for (a) and (b) (Excel sheet). (d) Mass spectrometry of immunoprecipitated Naa15 complexes (Excel sheet).
elife-65952-transrepform-v3.docx (110 kB)
Transparent reporting form
Figure 3—source data 1 Identification of a potential Naa10 homolog.
elife-65952-fig4-data1-v3.pdf (202 kB)
Figure 4—source data 1 Characterization of a potential Naa10 homolog.
elife-65952-fig5-data1-v3.pdf (28 kB)
Figure 5—source data 1 Generation of Naa12KO mice.
elife-65952-supp1-v3.docx (76 kB)
Tables related to genotypes, phenotypes and Mendelian ratios. (a) Genotypes of offspring from Naa10+/- female mice crossed to the Naa10+/Y male mice. Expected and observed Mendelian ratio of genotypes in offspring at E10.5, E13.5, E18.5 and adults from crosses of Naa10+/- female and Naa10+/Y male mice. The percentage of adult Naa10-/Y mice significantly decreases. (b) Genotypes of offspring from Naa10+/tm1a female mice crossed to the Naa10+/Y male mice. Expected and observed Mendelian ratio of genotypes in offspring at E10.5, E13.5, E18.5 and adults from crosses of Naa10+/tm1a female and Naa10+/Y male mice. The percentage of adults Naa10tm1a/Y mice significantly decreases. (c) Cervical fusion skeletal analyses in Naa10 knockout (KO) mice. (d) Matings and litter size analyses. (e) Genotypes of offspring from Naa12+/- female mice crossed to the Naa12+/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (f) Genotypes of offspring from Naa10+/- Naa12+/+ female mice crossed to the Naa10+/y Naa12+/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (g) Genotypes of offspring from Naa10+/- Naa12+/+ female mice crossed to the Naa10+/y Naa12-/- male mice. Expected and observed Mendelian ratio of genotypes in offspring from crosses. (h) Mendelian and observed offspring distributions from Naa10(+/Y); Naa12(+/-) male and Naa10(+/-); Naa12(+/-) female breeding. (i) Mendelian and observed offspring distributions from Naa10(+/Y); Naa12(-/-) male and Naa10(+/-); Naa12(+/-) female breeding. (j) Mendelian and observed postnatal offspring distributions from Naa10(+/Y); Naa12(+/-) male and Naa10(+/-); Naa12(+/+) female breeding. (k) Mendelian and observed postnatal offspring distributions from Naa10(+/Y); Naa12(-/-) male and Naa10(+/-); Naa12(+/+) female breeding. (l) Mendelian and observed age-specific offspring distributions from four crosses. (m) Mendelian and observed cumulative offspring distributions from all four crosses. (n) Mice analyzed by weighing, according to genotype. (o) Effects of Naa10 KO on growth rate of Naa10 mice on pure genetic background. (p) Effects of Naa10 and Naa12 KOs on growth rate on mixed genetic background.
elife-65952-supp2-v3.xlsx (8412 kB)
Mass spectrometry analyses. (a) N-termini detected in mouse embryonic fibroblasts (MEFs) (Excel sheet). (b) N-termini detected in all MEF samples (Excel sheet). (c) Header Key, showing the abbreviation meanings for (a) and (b) (Excel sheet). (d) Mass spectrometry of immunoprecipitated Naa15 complexes (Excel sheet).
elife-65952-transrepform-v3.docx (110 kB)
Transparent reporting form
