Journal
Elife
Publication Date
2021
Volume
10
First Page
e68457
Document Type
Open Access Publication
DOI
10.7554/eLife.68457
Rights and Permissions
eLife 2021;10:e68457 DOI: 10.7554/eLife.68457. © 2021, Avraham et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Recommended Citation
Avraham, Oshri; Feng, Rui; Ewan, Eric Edward; Rustenhoven, Justin; Zhao, Guoyan; and Cavalli, Valeria, "Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair." Elife. 10, e68457 (2021).
https://digitalcommons.wustl.edu/open_access_pubs/10869
elife-68457-fig1-data1-v1.xlsx (10 kB)
Figure 1—source data 1 Source files for scRNAseq analysis; top DEG for cell clustering and cluster counts.
elife-68457-fig1-data2-v1.xlsx (929 kB)
Figure 1—source data 2 Significant ligand-receptor interactions (p-value < 0.05).
elife-68457-fig2-data1-v1.xlsx (88658 kB)
Figure 2—source data 1 Source files for scRNAseq analysis; DEG in endothelial cells, pericytes and Schwann cells in response to peripheral and central injuries (FDR ≤ 0.05, fold-change ≥ 2), repair Schwann cell genes expression across injuries.
elife-68457-fig3-data1-v1.xlsx (50222 kB)
Figure 3—source data 1 Source files for scRNAseq analysis; Macrophage markers in the macrophage cluster, DEG in macrophages (FDR ≤ 0.05, fold-change ≥ 2) and MkKi67 expression.
elife-68457-fig4-data1-v1.xlsx (588 kB)
Figure 4—source data 1 Source files for scRNAseq analysis, flow cytometry additional experiments.
elife-68457-fig5-data1-v1.xlsx (35 kB)
Figure 5—source data 1 Source files for scRNAseq analysis; SGC marker genes expression, DEG in SGC subclusters (FDR ≤ 0.05, fold-change ≥2), Astrocytes and Schwann cells.
elife-68457-fig6-data1-v1.xlsx (794 kB)
Figure 6—source data 1 Source files for scRNAseq analysis; SGC subtypes injury marker genes, DEG in SGC subclusters in all injuries (FDR ≤ 0.05, fold-change ≥2), cluster distribution.
elife-68457-fig7-data1-v1.xlsx (50742 kB)
Figure 7—source data 1 Source files for scRNAseq analysis; DEG in SGC (FDR ≤ 0.05, fold-change ≥ 2), PPARαexpression in all cell types. Image analysis for axon regeneration.
elife-68457-sa2-fig1-v1.jpg (55 kB)
Enriched pathway (KEGG 2019) in SGC 3 days after SNC in our previous data set (2020) and new data set (2021).
elife-68457-video1.mp4 (9607 kB)
3D video of Imoonglia Glia.
elife-68457-transrepform1-v1.docx (246 kB)
Transparent reporting form
Figure 1—source data 1 Source files for scRNAseq analysis; top DEG for cell clustering and cluster counts.
elife-68457-fig1-data2-v1.xlsx (929 kB)
Figure 1—source data 2 Significant ligand-receptor interactions (p-value < 0.05).
elife-68457-fig2-data1-v1.xlsx (88658 kB)
Figure 2—source data 1 Source files for scRNAseq analysis; DEG in endothelial cells, pericytes and Schwann cells in response to peripheral and central injuries (FDR ≤ 0.05, fold-change ≥ 2), repair Schwann cell genes expression across injuries.
elife-68457-fig3-data1-v1.xlsx (50222 kB)
Figure 3—source data 1 Source files for scRNAseq analysis; Macrophage markers in the macrophage cluster, DEG in macrophages (FDR ≤ 0.05, fold-change ≥ 2) and MkKi67 expression.
elife-68457-fig4-data1-v1.xlsx (588 kB)
Figure 4—source data 1 Source files for scRNAseq analysis, flow cytometry additional experiments.
elife-68457-fig5-data1-v1.xlsx (35 kB)
Figure 5—source data 1 Source files for scRNAseq analysis; SGC marker genes expression, DEG in SGC subclusters (FDR ≤ 0.05, fold-change ≥2), Astrocytes and Schwann cells.
elife-68457-fig6-data1-v1.xlsx (794 kB)
Figure 6—source data 1 Source files for scRNAseq analysis; SGC subtypes injury marker genes, DEG in SGC subclusters in all injuries (FDR ≤ 0.05, fold-change ≥2), cluster distribution.
elife-68457-fig7-data1-v1.xlsx (50742 kB)
Figure 7—source data 1 Source files for scRNAseq analysis; DEG in SGC (FDR ≤ 0.05, fold-change ≥ 2), PPARαexpression in all cell types. Image analysis for axon regeneration.
elife-68457-sa2-fig1-v1.jpg (55 kB)
Enriched pathway (KEGG 2019) in SGC 3 days after SNC in our previous data set (2020) and new data set (2021).
elife-68457-video1.mp4 (9607 kB)
3D video of Imoonglia Glia.
elife-68457-transrepform1-v1.docx (246 kB)
Transparent reporting form
