Journal
Molecular Neurodegeneration
Publication Date
2016
Volume
11
Inclusive Pages
9
Document Type
Open Access Publication
DOI
10.1186/s13024-016-0073-8
Rights and Permissions
Reynolds et al. Molecular Neurodegeneration (2016) 11:9 DOI 10.1186/s13024-016-0073-8
Recommended Citation
Reynolds, Matthew R.; Singh, Itender; Azad, Tej D.; Holmes, Brandon B.; Verghese, Phillip B.; Dietrich, Hans H.; Diamond, Marc; Bu, Guojun; Han, Byung Hee; and Zipfel, Gregory J., "Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells." Molecular Neurodegeneration. 11, 9. (2016).
https://digitalcommons.wustl.edu/open_access_pubs/4530
s13024-016-0073-8-s1.docx (20 kB)
Heparin sulfate proteoglycan (HSPG) primer pairs for quantitative polymerase chain reaction (qPCR)
Supp2.jpeg (28 kB)
Soluble, monomeric Aβ 1-40 induces lipid oxidation in human VSMC
Supp3.jpeg (31 kB)
Apocynin does not impact baseline ROS production in VSMC
Supp4.jpeg (70 kB)
HSPG mitigates Aβ 1-40 -induced mitochondrial and cytosolic ROS production in VSMC under physiological oxygen concentration
Supp5.jpeg (75 kB)
Aβ 1-40 and Aβ 1-42 remain mostly in soluble, monomeric form after 30 minute incubation in L-15 media
Supp6.jpeg (90 kB)
HSPG subtype mRNA is present in primary human cerebral and transformed rat cerebral VSMC
Supp7.jpeg (42 kB)
Human VSMC cells from second source conform that HSPG mitigates Aβ 1-40 -induced mitochondrial and cytosolic ROS production in VSMC
Supp8.jpeg (56 kB)
Transformed rat cerebral VSMC behave similarly to human cerebral VSMC with respect to Aβ 1-40 -induced ROS production and mitigation of Aβ 1-40 -induced oxidative stress by pharmacological knockdown of HSPG with heparinase
Supp9.jpeg (44 kB)
Human VSMC cells also show Aβ 1-40 induces HSPG-mediated Ca 2+ influx
Heparin sulfate proteoglycan (HSPG) primer pairs for quantitative polymerase chain reaction (qPCR)
Supp2.jpeg (28 kB)
Soluble, monomeric Aβ 1-40 induces lipid oxidation in human VSMC
Supp3.jpeg (31 kB)
Apocynin does not impact baseline ROS production in VSMC
Supp4.jpeg (70 kB)
HSPG mitigates Aβ 1-40 -induced mitochondrial and cytosolic ROS production in VSMC under physiological oxygen concentration
Supp5.jpeg (75 kB)
Aβ 1-40 and Aβ 1-42 remain mostly in soluble, monomeric form after 30 minute incubation in L-15 media
Supp6.jpeg (90 kB)
HSPG subtype mRNA is present in primary human cerebral and transformed rat cerebral VSMC
Supp7.jpeg (42 kB)
Human VSMC cells from second source conform that HSPG mitigates Aβ 1-40 -induced mitochondrial and cytosolic ROS production in VSMC
Supp8.jpeg (56 kB)
Transformed rat cerebral VSMC behave similarly to human cerebral VSMC with respect to Aβ 1-40 -induced ROS production and mitigation of Aβ 1-40 -induced oxidative stress by pharmacological knockdown of HSPG with heparinase
Supp9.jpeg (44 kB)
Human VSMC cells also show Aβ 1-40 induces HSPG-mediated Ca 2+ influx
