Journal
Nature Communications
Publication Date
2016
Volume
7
Inclusive Pages
11901
Document Type
Open Access Publication
DOI
10.1038/ncomms11901
Rights and Permissions
E.S. Istvan, et al. 15 June 2016. A broad analysis of resistance development in the malaria parasite. Nature communications. Art. No. 11901. DOI: 10.1038/ncomms11901. http://www.nature.com/ncomms/2016/160615/ncomms11901/full/ncomms11901.html
Recommended Citation
Istvan, Eva S.; Goldberg, Daniel E.; and et al, "A broad analysis of resistance development in the malaria parasite." Nature Communications. 7, 11901. (2016).
https://digitalcommons.wustl.edu/open_access_pubs/5031
ncomms11901-s1.pdf (690 kB)
Supplementary Figures 1-5 and Supplementary Tables 1-2
ncomms11901-s2.xlsx (35 kB)
Summary of killing rate and selection success for compound panel. Compounds rate of killing profiles were determined based on a comparison analysis to fast (chloroquine - CQ), moderate (pyrimethamine - PYR), and slow (atovaquone - ATQ) compounds profiles. Time length to obtain mutants was analyzed, with 0-50 days, 50-125 days, and > 125 days corresponding to short, moderate, and long, respectively. Selection success was quantified by the generation of resistant mutations with an EC50 fold shift {greater than or equal to} 2. Compounds with successful selections are highlighted in green. Two compounds are noted as having NA selection success, as they were removed from the study due to cross-resistance with a strain generated in this study (MMV028895) or compound instability (MMV665824).
ncomms11901-s3.xlsx (50 kB)
Whole genome sequencing summary for MMV008149.
ncomms11901-s4.xlsx (74 kB)
Raw EC50 and Hill-Slope Data for strains analyzed in a hypoxanthine 48-hour assay with synchronized parasites in a 96-well format.
ncomms11901-s5.xlsx (82 kB)
Raw EC50 and Hill-Slope Data for strains analyzed in a SYBR Green 72-hour assay with synchronized parasites in a 384-well format.
ncomms11901-s6.xlsx (71 kB)
Supplementary Figures 1-5 and Supplementary Tables 1-2
ncomms11901-s2.xlsx (35 kB)
Summary of killing rate and selection success for compound panel. Compounds rate of killing profiles were determined based on a comparison analysis to fast (chloroquine - CQ), moderate (pyrimethamine - PYR), and slow (atovaquone - ATQ) compounds profiles. Time length to obtain mutants was analyzed, with 0-50 days, 50-125 days, and > 125 days corresponding to short, moderate, and long, respectively. Selection success was quantified by the generation of resistant mutations with an EC50 fold shift {greater than or equal to} 2. Compounds with successful selections are highlighted in green. Two compounds are noted as having NA selection success, as they were removed from the study due to cross-resistance with a strain generated in this study (MMV028895) or compound instability (MMV665824).
ncomms11901-s3.xlsx (50 kB)
Whole genome sequencing summary for MMV008149.
ncomms11901-s4.xlsx (74 kB)
Raw EC50 and Hill-Slope Data for strains analyzed in a hypoxanthine 48-hour assay with synchronized parasites in a 96-well format.
ncomms11901-s5.xlsx (82 kB)
Raw EC50 and Hill-Slope Data for strains analyzed in a SYBR Green 72-hour assay with synchronized parasites in a 384-well format.
ncomms11901-s6.xlsx (71 kB)
