Journal
G3
Publication Date
2017
Volume
7
Issue
1
Inclusive Pages
247-255
Document Type
Open Access Publication
DOI
10.1534/g3.116.036087
Rights and Permissions
Ho, NRY, Usmani AR, Yin Y, Ma L, Conrad DF (2017) Multiplex shRNA Screening of Germ Cell Development by in Vivo Transfection of Mouse Testis. G3, 7(1): 247-255 doi: 10.1534/g3.116.036087 Copyright © 2017 Ho et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Recommended Citation
Ho, Nicholas R.Y.; Usmani, Abul R.; Yin, Yan; Ma, Liang; and Conrad, Donald F., "Multiplex shRNA screening of germ cell development by in vivo transfection of mouse testis." G3. 7, 1. 247-255. (2017).
https://digitalcommons.wustl.edu/open_access_pubs/5492
Illustrated protocol for mouse testis injection.
FileS2.docx (42 kB)
Protocol for shRNA pool preparation and shRNA sequencing library preparation.
FileS3.zip (4 kB)
Code for analyzing shRNA count data.
FileS4.docx (153 kB)
Supplementary methods.
TableS1.xlsx (32 kB)
Summary of individual shRNA constructs used in this study.
TableS2.docx (91 kB)
Comparison of transfection rates.
TableS3.txt (3 kB)
Summary results of the pilot screen.
TableS4.txt (4 kB)
Summary results of the predicted genes screen.
TableS5.xlsx (150 kB)
shRNA read counts.
FigureS1.jpg (70 kB)
Overview of shRNA library prep.
FigureS2.png (393 kB)
Standard curve for Actin and shRNA qPCR primers.
FigureS3.png (1343 kB)
