Journal
Nature Communications
Publication Date
2017
Volume
8
Document Type
Open Access Publication
DOI
10.1038/ncomms14050
Rights and Permissions
Hammerling, B. C. et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat. Commun. 8, 14050 doi: 10.1038/ncomms14050 (2017). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Recommended Citation
Hammerling, Babette C.; Najor, Rita H.; Cortez, Melissa Q.; Shires, Sarah E.; Leon, Leonardo J.; Gonzalez, Eileen R.; Boassa, Daniela; Phan, Sébastien; Thor, Andrea; Jimenez, Rebecca E.; Li, Hong; Kitsis, Richard N.; Dorn II, Gerald W.; Sadoshima, Junichi; Ellisman, Mark H.; and Gustafsson, Åsa B., "A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance." Nature Communications. 8, (2017).
https://digitalcommons.wustl.edu/open_access_pubs/5558
Supplementary Figures
Supplementary Movie 1.mov (18619 kB)
Electron tomography of mitochondria sequestered inside endosomes in WT MEF. Four distinct endosomes all containing a single mitochondrion can be seen (one at bottom center, three at top center) after FCCP treatment. Single slice images of these puncta are found in Supplementary Fig. 5b,d. This movie and associated data can be accessed for downloading in the Cell Centered Database and Cell Image Library (http://www.cellimagelibrary.org/images?k=project_20381&simple_search=Search) under the project ID 20381.
Supplementary Movie 2.mov (29166 kB)
Electron tomography of mitochondria sequestered inside endosomes in Atg5-/- MEF with 3D model overlay. Two distinct endosomes both containing a single mitochondrion can be seen (top center and bottom center) after FCCP treatment. A third endosome, adjacent to the top endosome, can also be seen. Single slice images of these puncta are found in Figure 3B (top enlarged box), and Supplementary Figure 5i. In the top left-hand corner, a mitochondrion is in the process of being engulfed by an endosome. This event has been traced and 3D-modeled in Figure 4e. 3D Modeling clearly shows spherical mitochondria contained within early endosomes. This movie and associated data can be accessed for downloading in the Cell Centered Database and Cell Image Library (http://www.cellimagelibrary.org/images?k=project_20381&simple_search=Search) under the project ID 20381.
