Journal
Elife
Publication Date
3-27-2020
Volume
9
First Page
e55007
Document Type
Open Access Publication
DOI
10.7554/eLife.55007
Rights and Permissions
Lacin H, Williamson WR, Card GM, Skeath JB, Truman JW. Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS. Elife. 2020;9:e55007. Published 2020 Mar 27. doi:10.7554/eLife.55007. © 2020, Lacin et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Recommended Citation
Lacin, Haluk; Williamson, W. Ryan; Card, Gwyneth M.; Skeath, James B.; and Truman, James W., "Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS." Elife. 9, e55007 (2020).
https://digitalcommons.wustl.edu/open_access_pubs/9341
Figures and Figure Supplements.pdf (15131 kB)
elife-55007-video1.mp4 (1576 kB)
unc-4 mutant males (unc-4DBD /y) in open field
elife-55007-video2.mp4 (1408 kB)
Climbing activity of individual flies: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video3.mp4 (710 kB)
Climbing activity of group of flies: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video4.mp4 (900 kB)
Three-leg rubbing activity of individual flies; 2X slower: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video5.mp4 (1029 kB)
Tethered flight assay, 10X slower: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video6.mp4 (3070 kB)
Rescue of the flight take-off activity by GAL4/UAS system (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video7.mp4 (1526 kB)
Rescue of the flight take-off activity by a BAC transgene (uncDBD/y;; PBac(DC335) /+).
elife-55007-video8.mp4 (1481 kB)
Climbing activity of group of flies with the CNS and PNS specific deletion of unc-4: left, control (unc-4FRT /+; sca-GAL4/UAS-FLP.D); right, sca >unc-4FRT (unc-4FRT /y; sca-GAL4/UAS-FLP.D).
elife-55007-video9.mp4 (1922 kB)
Climbing activity of group of flies with the CNS specific deletion of unc-4: left, control (unc-4FRT /+; dpnEE-GAL4/UAS-FLP); right, dpnEE >unc-4FRT (unc-4FRT /y; dpnEE-GAL4/UAS-FLP).
elife-55007-video10.mp4 (160 kB)
Three-leg rubbing activity of individual flies with the chordotonal organ lineage specific deletion of unc-4: left, control (unc-4FRT /+; ato-GAL43.6/UAS-FLP); right, ato >unc-4FRT (unc-4FRT /y; ato-GAL43.6/UAS-FLP).
elife-55007-video11.mp4 (23801 kB)
Walking activity of individual flies in small-diameter tubes: left, control (unc-4FRT /+; ato-GAL43.6/UAS-FLP); middle, ato >unc-4FRT (unc-4FRT /y; ato-GAL43.6/UAS-FLP); right, unc-4 mutant (unc-4DBD /y).
elife-55007-video12.mp4 (13762 kB)
Magnified version of Video 11.
elife-55007-video13.mp4 (4379 kB)
The flight take-off behavior of flies with the 7B hemilineage specific deletion of unc-4 in response to banging the vial; 2X slower: left, control (5XUAS-FLP::PEST, unc-4FRT/+; dbxDBD/+; eyAD/+); right, NB-3–2-GAL4 > unc-4FRT (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video14.mp4 (370 kB)
The flight take-off behavior of flies with the 7B hemilineage specific deletion of unc-4 in response to visual looming stimuli; 200X slower: left, control (5XUAS-FLP::PEST, unc-4FRT/+; dbxDBD/+; eyAD/+); right, NB-3–2-GAL4 > unc-4FRT (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video15.mp4 (209 kB)
Flies with 7B specific unc-4 deletion initiating flight without take-off (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video16.mp4 (5540 kB)
The flight take-off behavior of flies with the ablated 7B hemilineage in response to banging the vial; 2X slower (UAS-hidAla5/+;; dbxDBD/+; eyAD/+).
elife-55007-supp1-v2.xlsx (11 kB)
Transparent Reporting Form.docx (246 kB)
elife-55007-video1.mp4 (1576 kB)
unc-4 mutant males (unc-4DBD /y) in open field
elife-55007-video2.mp4 (1408 kB)
Climbing activity of individual flies: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video3.mp4 (710 kB)
Climbing activity of group of flies: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video4.mp4 (900 kB)
Three-leg rubbing activity of individual flies; 2X slower: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video5.mp4 (1029 kB)
Tethered flight assay, 10X slower: left, unc-4 mutant males (unc-4DBD /y); right, rescued males (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video6.mp4 (3070 kB)
Rescue of the flight take-off activity by GAL4/UAS system (uncDBD/y; tubAD/10XUAS-IVS-myr::GFP; UAS-unc-4).
elife-55007-video7.mp4 (1526 kB)
Rescue of the flight take-off activity by a BAC transgene (uncDBD/y;; PBac(DC335) /+).
elife-55007-video8.mp4 (1481 kB)
Climbing activity of group of flies with the CNS and PNS specific deletion of unc-4: left, control (unc-4FRT /+; sca-GAL4/UAS-FLP.D); right, sca >unc-4FRT (unc-4FRT /y; sca-GAL4/UAS-FLP.D).
elife-55007-video9.mp4 (1922 kB)
Climbing activity of group of flies with the CNS specific deletion of unc-4: left, control (unc-4FRT /+; dpnEE-GAL4/UAS-FLP); right, dpnEE >unc-4FRT (unc-4FRT /y; dpnEE-GAL4/UAS-FLP).
elife-55007-video10.mp4 (160 kB)
Three-leg rubbing activity of individual flies with the chordotonal organ lineage specific deletion of unc-4: left, control (unc-4FRT /+; ato-GAL43.6/UAS-FLP); right, ato >unc-4FRT (unc-4FRT /y; ato-GAL43.6/UAS-FLP).
elife-55007-video11.mp4 (23801 kB)
Walking activity of individual flies in small-diameter tubes: left, control (unc-4FRT /+; ato-GAL43.6/UAS-FLP); middle, ato >unc-4FRT (unc-4FRT /y; ato-GAL43.6/UAS-FLP); right, unc-4 mutant (unc-4DBD /y).
elife-55007-video12.mp4 (13762 kB)
Magnified version of Video 11.
elife-55007-video13.mp4 (4379 kB)
The flight take-off behavior of flies with the 7B hemilineage specific deletion of unc-4 in response to banging the vial; 2X slower: left, control (5XUAS-FLP::PEST, unc-4FRT/+; dbxDBD/+; eyAD/+); right, NB-3–2-GAL4 > unc-4FRT (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video14.mp4 (370 kB)
The flight take-off behavior of flies with the 7B hemilineage specific deletion of unc-4 in response to visual looming stimuli; 200X slower: left, control (5XUAS-FLP::PEST, unc-4FRT/+; dbxDBD/+; eyAD/+); right, NB-3–2-GAL4 > unc-4FRT (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video15.mp4 (209 kB)
Flies with 7B specific unc-4 deletion initiating flight without take-off (5XUAS-FLP::PEST, unc-4FRT/y; dbxDBD/+; eyAD/+).
elife-55007-video16.mp4 (5540 kB)
The flight take-off behavior of flies with the ablated 7B hemilineage in response to banging the vial; 2X slower (UAS-hidAla5/+;; dbxDBD/+; eyAD/+).
elife-55007-supp1-v2.xlsx (11 kB)
Transparent Reporting Form.docx (246 kB)
