Journal
Elife
Publication Date
3-23-2020
Volume
9
First Page
e56095
Document Type
Open Access Publication
DOI
10.7554/eLife.56095
Rights and Permissions
Adapala NS, Swarnkar G, Arra M, et al. Inflammatory osteolysis is regulated by site-specific ISGylation of the scaffold protein NEMO. Elife. 2020;9:e56095. Published 2020 Mar 23. doi:10.7554/eLife.56095. © 2020, Adapala et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Recommended Citation
Adapala, Naga Suresh; Swarnkar, Gaurav; Arra, Manoj; Shen, Jie; Mbalaviele, Gabriel; Ke, Ke; and Abu-Amer, Yousef, "Inflammatory osteolysis is regulated by site-specific ISGylation of the scaffold protein NEMO." Elife. 9, e56095 (2020).
https://digitalcommons.wustl.edu/open_access_pubs/9342
elife-56095-fig1-data1-v2.pdf (1078 kB)
Western blot showing expression ofpMX-NEMOWT(NM) andpMX-NEMOmutants (NM-K270A, NEMO-D304N and NM-K319A).
elife-56095-fig1-data2-v2.xlsx (13 kB)
qPCR analysis for OC marker genes.
elife-56095-fig1-data3-v2.xlsx (10 kB)
RelA-luciferase activity.
elife-56095-fig2-data1-v2.xlsx (14 kB)
MicroCT analysis of bone from NM-WT and NM-KA mice.
elife-56095-fig2-data2-v2.xlsx (10 kB)
Serum concentration of TRAP and CTX.
elife-56095-fig3-data1-v2.xlsx (27 kB)
Serum concentration of cytokines from NM-WT and NM-KA mice measured by ELISA
elife-56095-fig3-data2-v2.xlsx (12 kB)
Representative qPCR analysis for OC marker genes
elife-56095-fig3-data3-v2.pdf (1078 kB)
Representative western-blot of p65 (phos-p65/p65 ratio) post RANKL stimulation in BMMs from NM-WT and NM-KA mice.
elife-56095-fig4-data1-v2.pdf (324 kB)
Western blot for LC3 using WES (protein simple).
elife-56095-fig4-data2-v2.xlsx (14 kB)
Quantification of LC3+ cells.
elife-56095-fig4-data3-v2.xlsx (10 kB)
LC3-GFP FACS analysis.
elife-56095-fig5-data1-v2.xlsx (13 kB)
NEMO-puncta quantification.
elife-56095-fig6-data1-v2.xlsx (280 kB)
Proteomic data from immunoprecipitated lysates from NM-WT compared with NM-KA BMMs.
elife-56095-fig6-data2-v2.pdf (679 kB)
Western blots for ISGylated proteins and free ISG15 in response to RANKL treatment.
elife-56095-fig6-data3-v2.xlsx (9 kB)
Osteoclast quantification from WT and ISG15-KO in vitro cultures.
elife-56095-fig7-data1-v2.xlsx (13 kB)
quantification of TRAP positive OCs.
elife-56095-fig7-data2-v2.xlsx (7903 kB)
LC3-GFP FACS analysis
elife-56095-fig7-data3-v2.xlsx (7914 kB)
Quantification of TRAP positive OCs.
elife-56095-fig7-data4-v2.xlsx (7920 kB)
Quantification of LC3 positive puncta in pre-OC cells shown in Figure 7—figure supplement 1.
elife-56095-fig7-data5-v2.pdf (688 kB)
Western blot for LC3 expression in preOC expressing different NEMO and ISG15 constructs.
Transparent Reporting Form.docx (246 kB)
