Journal
PeerJ
Publication Date
1-1-2020
Volume
8
First Page
e10118
Document Type
Open Access Publication
DOI
10.7717/peerj.10118
Rights and Permissions
Fasanello VJ, Liu P, Botero CA, Fay JC. 2020. High-throughput analysis of adaptation using barcoded strains of Saccharomyces cerevisiae. PeerJ 8:e10118 https://doi.org/10.7717/peerj.10118 © 2020 Fasanello et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
Recommended Citation
Fasanello, Vincent J; Liu, Ping; Botero, Carlos A; and Fay, Justin C, "High-throughput analysis of adaptation using barcoded strains of Saccharomyces cerevisiae." PeerJ. 8, e10118 (2020).
https://digitalcommons.wustl.edu/open_access_pubs/9792
Barcode cross-contamination rates for sequenced libraries
Supplemental_Figure_S2.pdf (23 kB)
Decreased contamination after reprocessing samples
Supplemental_Figure_S3.pdf (37 kB)
t-max by treatment visualization
Supplemental_Figure_S4.pdf (40 kB)
m-max by treatment visualization
Supplemental_Figure_S5.pdf (32 kB)
t-max-rate by treatment visualization
Supplemental_Figure_S6.pdf (39 kB)
m-max-rate by treatment visualization
Supplemental_Figure_S7.pdf (29 kB)
t-max-diff by treatment visualization
Supplemental_Figure_S8.pdf (38 kB)
m-max-diff by treatment visualization
Supplemental_Figure_S9.pdf (38 kB)
Total change in barcode abundance by treatment visualization
Supplemental_Table_S1.docx (23 kB)
Strain Construction, Yeast strains and inserted MOBY barcode sequences
Supplemental_Table_S2.docx (12 kB)
Treatments for 250-generation experimental evolutions
Supplemental_Table_S3.docx (16 kB)
Ion Proton sequencing primers
Supplemental_Table_S4.xlsx (16 kB)
Estimated fitness change in 250 generations evolution and significance of 91 barcoded yeast strains
Supplemental_Table_S5.xlsx (11 kB)
Data table of power calculations using error in the POC fitness assays data to support figure 3 panel A
Supplemental_Table_S6.xlsx (12 kB)
Data table of power calculations using error in the POC fitness assays data to support figure 3 panel B
Supplemental_Table_S7.xlsx (11 kB)
Data table of power calculations using error in the change in fitness data for the 250-generation experiment to support Fig. 3 panel A
Supplemental_Table_S8.xlsx (12 kB)
Data table of power calculations using error in the change in fitness data for the 250-generation experiment to support Fig. 3 panel B
Supplemental_Table_S9.xlsx (10 kB)
Change in fitness over 250 generations of experimental evolution
Supplemental_Table_S10.xlsx (22 kB)
Standard error in change in fitness across six treatments in 250 generations of experimental evolution
Supplemental_Table_S11.xlsx (11 kB)
Change in fitness across six treatments in 250 generations of experimental evolution
Supplemental_Table_S12.xlsx (10 kB)
t-max by treatment model results
Supplemental_Table_S13.xlsx (10 kB)
m-max by treatment model results
Supplemental_Table_S14.xlsx (10 kB)
t-max-rate by treatment model results
Supplemental_Table_S15.xlsx (10 kB)
m-max-rate by treatment model results
Supplemental_Table_S16.xlsx (10 kB)
t-max-diff by treatment model results
Supplemental_Table_S17.xlsx (10 kB)
m-max-diff by treatment model results
Supplemental_Table_S18.xlsx (10 kB)
Total change in barcode abundance by treatment model results
