Journal
Developmental biology
Publication Date
11-1-2019
Volume
455
Issue
1
First Page
42
Last Page
50
Document Type
Open Access Publication
DOI
10.1016/j.ydbio.2019.06.023
Rights and Permissions
Tang C, van den Bijgaart RJE, Looman MWG, Tel-Karthaus N, de Graaf AMA, Gilfillan S, Colonna M, Ansems M, Adema GJ. DC-SCRIPT deficiency delays mouse mammary gland development and branching morphogenesis. Dev Biol. 2019 Nov 1;455(1):42-50. doi: 10.1016/j.ydbio.2019.06.023. Epub 2019 Jun 29. PMID: 31265831. © 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
Recommended Citation
Tang, Chunling; van den Bijgaart, Renske J E; Looman, Maaike W G; Tel-Karthaus, Nina; de Graaf, Annemarie M A; Gilfillan, Susan; Colonna, Marco; Ansems, Marleen; and Adema, Gosse J, "DC-SCRIPT deficiency delays mouse mammary gland development and branching morphogenesis." Developmental biology. 455, 1. 42 - 50. (2019).
https://digitalcommons.wustl.edu/open_access_pubs/9876
Figure S1. Targeted disruption of DC-SCRIPT for generating DC-SCRIPT-/- mice
1-s2.0-S001216061930048X-mmc2.pdf (139 kB)
Figure S2. Gating strategy of flow cytometry analysis for segregating mammary epithelium (eFluor450-CD31-TER119-CD45-) from erythrocytes, dead cells, endothelial and immune cells.
1-s2.0-S001216061930048X-mmc3.pdf (92 kB)
Figure S3. Representative FACS analysis of EpCAM-CD49f versus CD24-CD49f to gate the luminal and the basal subpopulation from the same mammary gland of an 18 weeks old DC-SCRIPT+/+and DC-SCRIPT-/-mice.
1-s2.0-S001216061930048X-mmc4.pdf (323 kB)
Figure S4. Representative image of in vitro cultured mammary gland organoids on day 6 derived from 18 weeks old DC-SCRIPT+/+ and DC-SCRIPT-/- mice, scale bar 300μm.
