Journal

Developmental biology

Publication Date

11-1-2019

Volume

455

Issue

1

First Page

42

Last Page

50

Document Type

Open Access Publication

DOI

10.1016/j.ydbio.2019.06.023

1-s2.0-S001216061930048X-mmc1.pdf (76 kB)
Figure S1. Targeted disruption of DC-SCRIPT for generating DC-SCRIPT-/- mice

1-s2.0-S001216061930048X-mmc2.pdf (139 kB)
Figure S2. Gating strategy of flow cytometry analysis for segregating mammary epithelium (eFluor450-CD31-TER119-CD45-) from erythrocytes, dead cells, endothelial and immune cells.

1-s2.0-S001216061930048X-mmc3.pdf (92 kB)
Figure S3. Representative FACS analysis of EpCAM-CD49f versus CD24-CD49f to gate the luminal and the basal subpopulation from the same mammary gland of an 18 weeks old DC-SCRIPT+/+and DC-SCRIPT-/-mice.

1-s2.0-S001216061930048X-mmc4.pdf (323 kB)
Figure S4. Representative image of in vitro cultured mammary gland organoids on day 6 derived from 18 weeks old DC-SCRIPT+/+ and DC-SCRIPT-/- mice, scale bar 300μm.

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