Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs

Authors

Shan Tang, The Ohio State University
Xue Wu, The Ohio State University
Jinghui Liu, University of Kentucky
Qiongsi Zhang, University of Kentucky
Xinyi Wang, University of Kentucky
Shuai Shao, The Ohio State University
Birkan Gokbag, The Ohio State University
Kunjie Fan, The Ohio State University
Xiaoqi Liu, University of Kentucky
Fuhai Li, Washington University School of Medicine in St. Louis
Lijun Cheng, The Ohio State University
Lang Li, The Ohio State University

Journal

STAR Protocols

Publication Date

9-16-2022

Volume

3

Issue

3

First Page

101556

Document Type

Open Access Publication

DOI

10.1016/j.xpro.2022.101556

Rights and Permissions

Tang S, Wu X, Liu J, Zhang Q, Wang X, Shao S, Gokbag B, Fan K, Liu X, Li F, Cheng L, Li L. Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs. STAR Protoc. 2022 Aug 24;3(3):101556. doi: 10.1016/j.xpro.2022.101556. Copyright 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Recommended Citation

Tang, Shan; Wu, Xue; Liu, Jinghui; Zhang, Qiongsi; Wang, Xinyi; Shao, Shuai; Gokbag, Birkan; Fan, Kunjie; Liu, Xiaoqi; Li, Fuhai; Cheng, Lijun; and Li, Lang, "Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs." STAR Protocols. 3, 3. 101556 (2022).
https://digitalcommons.wustl.edu/oa_4/2725

Department

ICTS (Institute of Clinical and Translational Sciences)

Additional Links

Supplemental material is available for this article at publisher site.