Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody

Authors

Justin O'Leary, Mayo Clinic
Ana-Caroline Raulin, Mayo Clinic
Zonghua Li, Mayo Clinic
Yuka Martens, Mayo Clinic
Yasuteru Inoue, Mayo Clinic
Michael R Strickland, Washington University School of Medicine in St. Louis
Xianlin Han, University of Texas Health Science Center at San Antonio
David M Holtzman, Washington University School of Medicine in St. Louis
Guojun Bu, Mayo Clinic
Na Zhao, Mayo Clinic

Journal

STAR Protocols

Publication Date

6-7-2023

Volume

4

Issue

2

First Page

102271

Document Type

Open Access Publication

DOI

10.1016/j.xpro.2023.102271

Rights and Permissions

O'Leary J, Raulin AC, Li Z, Martens Y, Inoue Y, Strickland MR, Han X, Holtzman DM, Bu G, Zhao N. Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody. STAR Protoc. 2023 Jun 7;4(2):102271. doi: 10.1016/j.xpro.2023.102271. Copyright 2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Recommended Citation

O'Leary, Justin; Raulin, Ana-Caroline; Li, Zonghua; Martens, Yuka; Inoue, Yasuteru; Strickland, Michael R; Han, Xianlin; Holtzman, David M; Bu, Guojun; and Zhao, Na, "Standardized immunoprecipitation protocol for efficient isolation of native apolipoprotein E particles utilizing HJ15.4 monoclonal antibody." STAR Protocols. 4, 2. 102271 (2023).
https://digitalcommons.wustl.edu/oa_4/2789

Department

ICTS (Institute of Clinical and Translational Sciences)