Efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors with unbiased whole-genome characterization by LOCK-seq

Authors

• Monica F Sentmanat, Washington University School of Medicine in St. Louis
• Zi Teng Wang, Washington University School of Medicine in St. Louis
• Evguenia Kouranova, Washington University School of Medicine in St. Louis
• Samuel T Peters, Washington University School of Medicine in St. Louis
• Wan Ching Chan, Washington University School of Medicine in St. Louis
• Jed Lin, Washington University School of Medicine in St. Louis
• Yong Miao, Washington University School of Medicine in St. Louis
• J Michael White, Washington University School of Medicine in St. Louis
• Mia Wallace, Washington University School of Medicine in St. Louis
• Xiaoxia Cui, Washington University School of Medicine in St. Louis

Journal

Nucleic acids research

Publication Date

3-19-2026

Volume

54

Issue

6

First Page

gkag237

Document Type

Open Access Publication

DOI

10.1093/nar/gkag237

Rights and Permissions

Monica F Sentmanat, Zi Teng Wang, Evguenia Kouranova, Samuel T Peters, Wan Ching Chan, Jed Lin, Yong Miao, J Michael White, Mia Wallace, Xiaoxia Cui, Efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors with unbiased whole-genome characterization by LOCK-seq, Nucleic Acids Research, Volume 54, Issue 6, 13 April 2026, gkag237, https://doi.org/10.1093/nar/gkag237. https://academic.oup.com/nar/article/54/6/gkag237/8625898?login=true. © The Author(s) 2026. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Recommended Citation

Sentmanat, Monica F; Wang, Zi Teng; Kouranova, Evguenia; Peters, Samuel T; Chan, Wan Ching; Lin, Jed; Miao, Yong; White, J Michael; Wallace, Mia; and Cui, Xiaoxia, "Efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors with unbiased whole-genome characterization by LOCK-seq." Nucleic acids research. 54, 6. gkag237 (2026).
https://digitalcommons.wustl.edu/oa_4/6522

Department

ICTS (Institute of Clinical and Translational Sciences)

Additional Links

Supplemental material is available for this article at publisher site.